Phospho flow antibodies

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August undefined, 2024

WebEach phospho-specific antibody has been tested in three different flow cytometry buffer systems: IC Fixation and Permeabilization, Foxp3/Transcription Factor Buffer, and IC Fixation Buffer/Methanol. The recommended buffer system(s) will be noted on the … Find manuals and technical documents, get account and ordering help, browse … Flow Cytometry Support Center—Find technical support recommendations for … The Foxp3 Transcription Factor Staining Buffer Set has been formulated and … WebFor example, staining intracellular targets for phospho-flow cytometry experiments requires that you fix and permeabilize your cells before staining. However, fixation (fix) and permeabilization (perm) protocols can alter the interaction between epitope and antibody.

Phospho-Specific Antibodies for Flow Cytometry

WebRabbit recombinant monoclonal p38 (phospho T180 + Y182) antibody [P38T180Y182-E3] conjugated to PE. Validated in Flow Cyt and tested in Rat. Immunogen corresponding to synthetic peptide. WebJun 5, 2015 · The eight phospho-specific antibodies were chosen to represent major signaling nodes responsive to cytokine, TLR, and antigen receptor signaling. This antibody … impacts of bushfires for kids

Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb (PE Conjugate)

WebPhospho-specific antibody flow cytometric analysis of phosphorylated proteins allows researchers a quick and effective way to measure signaling cascades in individual cells. A … WebMonoclonal Antibody for studying Bad (Ser99) phosphate. Cited in 1 publication. Highly specific and rigorously validated in-house, Phospho-Bad (Ser136) (5D8) Mouse Monoclonal Antibody (CST #5282) is ready to ship. WebThis product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility. - Improved sensitivity and specificity. - Long-term security of supply. - … impacts of burning plastics

Rabbit anti-Phospho-SMC1A-S957 Antibody RayBiotech

Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (cupk43k), PE …

WebJan 1, 2012 · Antibodies: 5. Anti-human and mouse phospho-STAT3 (pY705) mouse antibody conjugated to phycoerythrin (PE) (BD/Phosflow, catalogue number: 612569). 6. Anti-human and mouse phospho-STAT5a,b (pY694) mouse antibody conjugated to Alexa Fluor ® 488 (BD/Phosflow, catalogue number: 612598). 7. WebKnockout Tested Rabbit recombinant monoclonal ZFP36L1 (phospho S92) antibody [EPR19926] - BSA and Azide free. Validated in WB, Dot and tested in Mouse, Human. list three characteristics of a virusWebPhospho-FLT3 (Tyr591) Antibody detects endogenous FLT3 only when phosphorylated at tyrosine 591. The antibody does not cross-react with other FLT family members. It may cross-react with some tyrosine phosphorylated proteins. Species Reactivity: Human, Mouse Source / Purification impacts of caffeine on the body

"WebPhospho-NFkB p65 (Ser536) Antibody Applications: WB, FCM, ICC, IP Reactivity: Hu, Ms, Rt, Ca, Hm, NHPr, Pg Conjugate/Tag: Unconjugated Quantity: 100 µL Citations: (25) Supplier Page Sign In or Register to view pricing Compare Product + See More (4) View All (18) Biorbyt NFKB p65 antibody Applications: WB, ELISA, FCM, ICC, IF, IHC-fr, IHC-p " - Phospho flow antibodies

Phospho flow antibodies

Anti-NFAT1 (phospho S54) antibody (ab200819) Abcam

WebFlow cytometry offers a quick and effective way to measure signaling at a specific time point in individual cells. The staining methods for detecting signaling molecules and … WebProtocol Outline. Prepare all reagents and samples as instructed in the manual. Add 100 µl of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 µl …

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WebPhosphoflow is a specialized application of flow cytometry that enables researchers to examine the phosphorylation status of specific proteins that govern cellular responses … WebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR1899 (2)] to JAK1 (phospho Y1034 + Y1035) - BSA and Azide free. Suitable for: ELISA, Dot blot, WB, IHC-P. Reacts with: Human.

WebPrimary Anti-ULK1 (phospho S556) antibody (ab203207) Primary Anti-AMPK gamma 1 antibody [Y308] (ab32508) Primary Anti-Acetyl Coenzyme A Carboxylase (phospho S79) antibody [EP1885Y] (ab68191) View more … WebThis antibody conjugate is suitable for intracellular staining of human cell lines and peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD Phosflow™ permeabilization buffers may be used. Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test.

WebPE anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody - ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ... Each lot of this … WebKey features and details. Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply. Rabbit monoclonal [EPR1899 (2)] to JAK1 …

WebThe antibody pair provided in this kit recognizes human Phospho-EGFR (pTyr1045). Storage Stability. Rapidly measure phosphorylated protein in lysates. Screen numerous different cell lysates without performing a Western Blot analysis. Minimal hands-on time, convenient, and non-radioactive material.

WebRabbit recombinant monoclonal PKC (phospho T514) antibody [PKCgT514-PF4]. Validated in WB, Flow Cyt and tested in Mouse, Rat, Human. Immunogen corresponding to synthetic peptide. impacts of change in businessWebFeb 26, 2024 · p-Ser Antibody (16B4) is an IgM κ mouse monoclonal p-Ser antibody (also designated phosphorylated serine antibody, phospho serine antibody, or p-serine antibody) that detects the p-Ser protein of mouse, rat, human and canine origin by WB and IP. p-Ser Antibody (16B4) is available as the non-conjugated anti-p-Ser antibody. list three chemical propertiesWebFlow Cytometry - APC Anti-p38 (phospho T180 + Y182) antibody [P38T180Y182-E3] (ab278728) Flow cytometric analysis of C6 cells secondary antibody only negative control (blue) or untreated (red) or treated with staurosporine (green) using ab278728. impacts of caffeine during pregnancyWebPrimary Antibodies (9010) Secondary Antibodies (76) Antibody Conjugates (1994) Activators & Inhibitors (224) Buffers & Dyes (108) Cellular Assay Kits (118) ChIP Kits & Reagents (156) CUT&RUN Kits & Reagents (15) Cytokines & Growth Factors (215) ELISA Kits (513) Experimental Controls (248) Flow Cytometry Kits & Reagents (9) Proteomic Analysis … impacts of child obesityWebPhospho-flow has been applied to numerous areas of biology, including antigenic stimulation and microbial challenge, high-throughput and high-content drug discovery, … impacts of childhood trauma on adultsWebAliquot an optimal concentration of phosphospecific fluorochrome-conjugated antibody to each tube. Incubate at room temperature for 30 minutes in the dark. Wash once with 2 mL … list three characteristics of lymphocytesWebPolyclonal Antibody for studying PLCg1 (Tyr783) phosphate. Cited in 265 publications. Validated for Western Blotting. Available in 3 sizes. Highly specific and rigorously … impacts of chernobyl