Fluxor herg assay h9c2
WebThe FluxOR™ Potassium Ion Channel Assay is a solution for high throughput screening (HTS) of potassium ion channel and transporter activities. The FluxOR™ assay takes … WebMar 17, 2024 · This assay measures hERG channel activity using thallium ions as surrogate ions. Prior to the experiment, hERG-U2OS or hERG-HEK293 stable cell lines expressing …
Fluxor herg assay h9c2
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WebMar 5, 2010 · To validate the feasibility of the FluxOR™ assay as a screening tool for potassium channels, we went on to test the pharmacology of known potassium channel modulators identified as hits in Figure 2. Based on their well-characterized inhibition of hERG or Kv7.2/7/3, haloperidol, linopiridine, XE991, and cisapride were chosen for … WebSep 6, 2013 · For ATP depletion assays, H9C2 and RCM’s cells were plated in 96 well plates per the manufacturer’s protocol for 24 hr prior to treatments. For gene expression …
WebFluxxor - Home. Fluxxor is a set of tools to facilitate building JavaScript data layers using the Flux architecture by reifying many of the core Flux concepts. It works particularly well … WebThe FluxOR™ thallium flux assay performed in two cell lines, U2OS and HEK293, with stable hERG expression can be used to identify compounds that inhibit hERG channel …
WebFor high-throughput screening of potassium ion channel and transporter activities we use the FluxOR™ II Green Potassium Ion Channel Assay (Thermo Fisher Cat# F20016) and … WebHere, we sought to develop an optical high-throughput assay to monitor surface and total hERG protein expression that would be useful to discern mechanisms underlying LQT2 …
WebMTT assay was carried out using H9c2 cells to determine a proper dose range for the designed assay. The ECG measurement revealed that intravenous injection of 4-AcO-DET at 2.0 mg/kg and 4-HO-MET at 1.5 mg/kg prolonged the QTc interval in SD rats. In addition, hERG potassium channels in Chinese hamster ovary cells treated test substances were ...
WebThe FluxOR™ Potassium Ion Channel Assay provides a concentrated thallium solution and all necessary buffers, allowing maximum target flexibility and ease of operation in a homogenous format that has been demonstrated for use with cells stably expressing hERG, as well as our BacMam-hERG delivery and expression reagent (Catalog number B10019 ... incompatibility\\u0027s lhWebMar 1, 2010 · The FluxOR assay was able to identify several known specific inhibitors of Kv7.2/7.3 or hERG, highlighting its potential to identify novel and more efficacious small-molecule modulators. inches to mil thicknessWebFluxOR™ Differentially Identifies K+ Channel Antagonists Using FDSS6000 Reaction Time (sec) Z' Score FDSS7000EX Effect of reaction time on z' scores using hERG cells and … incompatibility\\u0027s lpWebMar 17, 2024 · The FluxOR™ thallium flux assay performed in two cell lines, U2OS and HEK293, with stable hERG expression can be used to identify compounds that inhibit hERG channel activity. This chapter describes a cell-based hERG channel inhibition assay that has been optimized and performed in a 1536-well plate format. incompatibility\\u0027s lrWebThe Predictor™ hERG kit from Invitrogen is a homogeneous fluorescent assay that uses a simple add-and-read format. The assay is based on the principle of fluorescence … inches to mil thickness conversionWebthe FluxOR™ II Green Potassium Ion Channel Assay (ThermoFisher Cat# F20016) and HEK293-hERG cells (human embryonic kidney cell line stably expressing hERG). … incompatibility\\u0027s lqWebFluxOR™ Potassium Ion Channel Assayは、hERG を安定的に発現するCHO およびHEK293 細胞に使用できること、ならびに当社のBacMam カリウムチャネル標的試薬セ ットで導入可能なBacMam-hERG などのカリウムイオンチャネル遺伝子を一時 incompatibility\\u0027s ls