WebMar 5, 2003 · 2.6. Synthesis of biotin–PEG–NHS. Biotin–PEG–COOH (15 mg, 12 μmol) was dissolved in DMF (300 μl) and pyridine (200 μl) was added.TSTU (7.2 mg, 24 μmol; [25]) was separately dissolved in DMF (100 μl) and added with stirring. After 2 h of stirring at RT the mixture was frozen in liquid N 2, vacuum (1–10 Pa) was applied, and upon … WebPEG NHS ester linkers have been widely usded for crosslinking or labeling peptides and proteins. They readily react with primary amine groups at the N-terminus of polypeptide chains and lysine amino groups. These primary amines exist predominantly on the outside surfaces of protein and are readily accessible to NHS ester for bioconjugations. In ...
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WebCAS NO. 35013-72-0. Description. Biotin-NHS ester consists of the native biotin valeric acid group only. Its membrane permeability allows it to be used for intracellular labeling. NHS group can react efficiently with primary amino groups (-NH2) to form stable, irreversible amide bonds. Molecular Weight. WebBiotinylation reagents are very popular labeling reagents in biochemistry and they are commonly used for covalent coupling of biotin to a protein, DNA or other molecules. The non-covalent interaction of the biotin-avidin is very useful for purifying or detecting proteins of interest. BroadPharm provides a wide selection of biotinylation reagents, which can … green dry cleaning
Biotin-NHS ester - Creative Biolabs
WebBiotin PEG Fluorescein, Biotin-PEG-FITC, MW 20000. $385. Desthiobiotin PEG derivatives and Iminobiotin PEG derivatives, call for details. Vinyl sulfone PEG Biotin, phospholipid PEG Biotin, Cy3/Cy5 PEG Biotin … WebNHS-dPEG ®4 -biotin is used extensively to label molecules that contain a free amine with biotin. The single molecular weight NHS-dPEG ®4 -biotin has a long spacer of precise … WebJenKem Technology is capable of development and synthesis of a wide range of GMP grade PEG derivatives under ISO 9001 and ISO 13485 certified quality management system, following ICH Q7 guidelines for GMP manufacture. For detailed information about JenKem Technology’s custom PEG synthesis services, please contact us at … green dry cleaning process